Anterior cingulated cortex modulating the nociception of spinal in CCI rats
- VernacularTitle:慢性坐骨神经结扎大鼠前扣带皮层对脊髓痛觉信息传递的调节
- Author:
Jing XU
;
Yugang LU
;
Weifeng YU
- Publication Type:Journal Article
- Keywords:
Anterior cingulate cortex;
Spinal Cord;
cAMP-response element binding protein;
Extracellular regulated protein kinases
- From:
The Journal of Clinical Anesthesiology
2016;32(4):393-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe how the ACC transmits nociceptive information and how it regulates spinal noceciption.Methods A total of 32 male SD rats were randomly divided into four groups:Sham group,CCI group,ACC group,and AP-5 group.After light-dark transition test, forced swimming test (FST),paw withdrawal mechanical threshold (PWMT),paw withdrawal ther-mal latency (PWTL)had been measured,the rats were finally anesthetized,then ACC and the spinal cord was rapidly removed,the expression of cAMP-response element binding protein (pCREB)and extracellular regulated protein kinases (pERK)were measured by Western blot.Results Compared with the sham group,the PWMT of the CCI rats were significantly decreased,rats spent less time in the light compartment and number of transition were decreased (P <0.01 ).The immobility time in FST were also significantly prolonged (P <0.01).After AP-5 injected in bilateral ACC 13 days after CCI operation,the PWMT of the CCI rats were significantly increased,rats spent more time in the light compartment and number of transition were increased (P <0.01).The immobility time in FST were also significantly shortened (P <0.01).Compared with sham group,the expression of pCREB, pERK increased significantly in ACC in CCI group (P <0.01).The expression of pCREB,pERK in spinal cord was increased in CCI group and decreased in AP-5 group (P <0.01 ).Conclusion ACC facilitate the spinal nociception in a descending mode.
