Differentially expressed serum proteins in oral lichen planus patients before and after treated with Huashi Xingyu Qingre Decoction
10.16571/j.cnki.1008-8199.2016.04.010
- VernacularTitle:化湿行瘀清热方剂治疗口腔扁平苔藓前后血清中差异蛋白的初步研究
- Author:
Jian LIU
;
Meijie ZHANG
;
Tiejun LIU
;
Yangyang ZHANG
;
Yanzhi XU
- Publication Type:Journal Article
- Keywords:
Oral lichen planus;
Huashi Xingyu Qingre decoction;
Serum protein;
2D DIGE;
LC-MS;
Western blot
- From:
Journal of Medical Postgraduates
2016;29(4):384-387
- CountryChina
- Language:Chinese
-
Abstract:
Objective Oral lichen planus ( OLP) is a common complaint in the oral mucosa , for which there is no definite therapy hitherto.This article aimed to investigate the possible effect of Huashi Xingyu Qingre Decoction (HXQD) on OLP. Methods This study included 30 randomly selected cases of OLP treated with HXQD .Fasting venous blood and total serum protein were obtained before and after medication for screening and identification of OLP-related differential proteins by two-dimensional fluorescence differ-ence gel electrophoresis (2D DIGE) and liquid chromatography-mass spectrometry (LC-MS), followed by Western blot validation . Results Haptoglobin , antithrombin Ⅲ, C1 complement , and vitamin D binding protein were differentially expressed in the serum of the OLP patients before and after treated with HXQD .Compared with the baseline , the expression of haptoglobin was significantly in-creased (103.086 ±27.536 vs 159.704 ±24.228, P<0.05) while that of antithrombin Ⅲ remarkably decreased after treatment (150.00 ±54.04 vs 98.00 ±28.04, P<0.05). Conclusion Haptoglobin, antithrombin Ⅲ, C1 complement, and vitamin D binding protein are differentially expressed in the serum of OLP patients before and after HXQD medication , which may be associated with the development and progression of OLP .
