The effects of miR-20a on the osteogenic differentiation potential of the inflammatory PDLSCs
10.3969/j.issn.1001-3733.2016.02.007
- VernacularTitle:miR-20a 对人炎症牙周膜干细胞成骨分化能力的影响
- Author:
Qianjuan YANG
;
Wenjia LIU
;
Wenyue CHANG
;
Xiaorui GUO
;
Hong WANG
;
Qi CHEN
;
Qun LU
- Publication Type:Journal Article
- Keywords:
miR-20a;
Periodontal ligament stem cells(PDLSCs);
Osteogenic differentiation potential
- From:
Journal of Practical Stomatology
2016;32(2):186-189
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of miR-20a on the osteogenic differentiation potential of inflammatory periodontal liga-ment cells(IPDLSCs).Methods:Cells were isolated and cultured from the healthy and inflammatory periodontal ligament samples (HPDLSCs and IPDLSCs)respectively.miR-20a expression was analyzed by qRT-PCR.Alizarin red staining,Western blot and PCR were used to evaluate the osteogenic differentiation potential of IPDLSCs after transient transinfection of miR-20a mimics or inhib-itor.Results:miR-20a expression in IPDLSCs was lower than that in HPDLSCs,and the osteogenic differentiation potential of IP-DLSCs were promoted by miR-20a mimics,and reduced by miR-20a inhibitor.Conclusion:The miR-20a in IPDLSCs was down reg-ulated.miR-20a can promote the osteogenic differentiation potential of IPDLSCs.
