Targeted inhibition of Rad50 increases the radiosensitivity of head and neck squamous cell carcinoma cells:A pilot study

VernacularTitle:靶向抑制 Rad50提高头颈部鳞癌细胞放疗敏感性的初步研究
Author: Jun ZHENG ; Xiaoming WANG ; Chanyuan DU ; Mengqi ZHANG ; Hui XUE ; Ke WANG ; Gang LI ; Moyi SUN ; Xiufeng BAI
Publication Type:Journal Article
Keywords: Rad50; Targeted inhibition; Head and neck squamous cell carcinoma cell lines; Radiosensitivity
From: Journal of Practical Stomatology 2016;32(2):190-195
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effects of Rad50-targeted inhibition on the radiosensitivity of head and neck squamous cell carcinoma cells.Methods:Rad50 in Tca8113 and OSCC-15 cells was down-regulated by siRNA.Then the cells were treated by a small dose of radiation(2 Gy),Western blot was used to detect the expression of Rad50 protein.Comet assay was used to evaluate the DNA double strand breaks(DSBs).Colony survival assay was performed to detect the survival rate of the cells.Telomere FISH was performed to assess the telomere length in the cells.Results:Target siRNA inhibited Rad50 protein expression in Tca8113 and OSCC-15 cells. siRNA combined with the radiation of 2 Gy produced more DSBs,decreased the proliferation and shortened the nucleus telomere length of the cells more than radiation treatment alone.Conclusion:The targeted inhibition of Rad50 may increase the radiosensitivity of Tca8113 and OSCC-15 cells.