Isolation,cultivation and identification of dental pulp stem cells from canine
10.3969/j.issn.1001-3733.2016.01.021
- VernacularTitle:犬牙髓干细胞的分离培养与鉴定
- Author:
Jun GUO
;
Lu HE
;
Jian YANG
- Publication Type:Journal Article
- Keywords:
Canine;
Dental pulp stem cells;
Osteogenic differentiation;
Adipogenic differentiation
- From:
Journal of Practical Stomatology
2016;32(1):99-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To culture canine dental pulp stem cells(cDPSCs)in vitro.Methods:Canine pulp cells were isolated and cultured by enzyme digestion and explanted tissue culture respectively.Cell morphology was observed under phase-contrast micro-scope.The clone forming unit(CFU)of the cells was examined by plate clone formation assay.Cell markers and protein-expression were examined by flow cytometry(FC)and immunofluorescence.Odontogenic and adipogenic potential were evaluated by alizarin red staining and oil red O staining.Results:Short spindle fibroblast-like and steadily growing cells were obtained by both methods.The clone assay showed that CFU was 1 5.1 7% ±2.79%.FC observasion showed that the CD90,STRO-1 and CD24 positive cells were 24.43% ±7.1 0%,20.67% ±1 .42% and 2.03% ±0.06% respectively,but CD34 was negative.Immunofluorescence analysis showed positive expression of Nestin,Vimentin,weak expression of ALP and negative expression of DSP of the cells.Differentiation ex-periment confirmed the odontogenic and adipogenic differentiation potential of the cells.Conclusion:cDPSCs can be cultured in vitro.