Regulation of the proliferation and differentiation of mice bone marrow mesenchymal stem cells by CKIP-1 in vitro

VernacularTitle:CKIP-1对骨髓间充质干细胞体外增殖和分化能力的影响
Author: Xiaoguang TIAN ; Shuning SHEN ; Yinzhong DUAN ; Min YU ; Lei WANG
Publication Type:Journal Article
Keywords: CKIP-1 knockout; Bone marrow mesenchymal stem cells(BMSCs); Osteogenic differentiation; Adip-genic differentiation
From: Journal of Practical Stomatology 2016;32(1):10-14
CountryChina
Language:Chinese
Abstract: Objective:To investigate the regulation of proliferation and differentiation of bone marrow mesenchymal stem cells(BM-SCs)by CKIP-1 in vitro.Methods:BMSCs from CKIP-1 nock out(KO)and wild type(WT)C57 mice were isolated and cultured u-sing adherence method in vitro.BMSCs of the 3rd passage were induced to osteogenic and adipgenic differentiation.Cell proliferation was examined by MTT assay.Cell surface markers were tested by FCM.The osteogenic and adipogenic differentiation was studied by alkaline phosphatase (ALP)staining,alizarin red staining and oil red O staining.Results:The proliferation and cell marke expression of the 2 groups were similar.ALP staining of KO group was strong than that of WT group after osteogenic induction.Alizarin red stai-ning showed that there were more mineralized nodules in BMSCs of KO group than in those of WT group.Oil red O staining of KO mice BMSCs was stronger than that of WT.Conclusion:CKIP-1 deficiency can enhance the osteogenic and adipogenic differentiation without influence on the proliferation of BMSCs.