The impact of endothelial progenitor cells on the osteogenesis of bone marrow mesenchymal stem cell sheet-implant complex

VernacularTitle:内皮祖细胞对骨髓间充质干细胞－种植体复合物成骨能力的影响
Author: Huan LIU ; Wei ZHOU ; Nan REN ; Qin WU ; Rui XIE ; Tian QIN ; Yimin ZHAO
Publication Type:Journal Article
Keywords: Endothelial progenitor cells(EPCs); Bone marrow mesenchymal stem cells(BMSCs); Cell sheet; Implant
From: Journal of Practical Stomatology 2016;32(2):155-160
CountryChina
Language:Chinese
Abstract: Objective:To study the effects of endothelial progenitor cells(EPCs)on the osteogenesis of bone marrow mesenchymal stem cell (BMSCs)sheet-implant complex.Methods:EPCs were added to the BMSC sheets,and the expression of osteogenesis-relat-ed genes was examined by real time PCR.Cell sheets were wrapped around implants to construct cell sheet-implant complexes and the complexes were subcutaneously transplanted into SCID mice.The complexes were harvested 8 weeks after operation and observed by micro-CT and histological examination.Results:The BMSC sheet with EPCs showed higher expression of Runx2,ALP,BMP2 and VEGF in the in vitro test;higher bone volume ratio,greater amount of new bone tissue and higher expression of Runx2 and BMP2 in the in vivo test.Conclusion:EPCs can improve the osteogenesis of BMSC sheet-implant complex.