The impact of endothelial progenitor cells on the osteogenesis of bone marrow mesenchymal stem cell sheet-implant complex
10.3969/j.issn.1001-3733.2016.02.001
- VernacularTitle:内皮祖细胞对骨髓间充质干细胞-种植体复合物成骨能力的影响
- Author:
Huan LIU
;
Wei ZHOU
;
Nan REN
;
Qin WU
;
Rui XIE
;
Tian QIN
;
Yimin ZHAO
- Publication Type:Journal Article
- Keywords:
Endothelial progenitor cells(EPCs);
Bone marrow mesenchymal stem cells(BMSCs);
Cell sheet;
Implant
- From:
Journal of Practical Stomatology
2016;32(2):155-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of endothelial progenitor cells(EPCs)on the osteogenesis of bone marrow mesenchymal stem cell (BMSCs)sheet-implant complex.Methods:EPCs were added to the BMSC sheets,and the expression of osteogenesis-relat-ed genes was examined by real time PCR.Cell sheets were wrapped around implants to construct cell sheet-implant complexes and the complexes were subcutaneously transplanted into SCID mice.The complexes were harvested 8 weeks after operation and observed by micro-CT and histological examination.Results:The BMSC sheet with EPCs showed higher expression of Runx2,ALP,BMP2 and VEGF in the in vitro test;higher bone volume ratio,greater amount of new bone tissue and higher expression of Runx2 and BMP2 in the in vivo test.Conclusion:EPCs can improve the osteogenesis of BMSC sheet-implant complex.