Double-layer nanoparticles loaded with rhBMP-2 and SDF-1:Preparation,characterization and drug re-lease in vitro
10.3969/j.issn.1001-3733.2016.02.002
- VernacularTitle:包载 rhBMP-2和 SDF-1的双层纳米微球的构建及体外释药研究
- Author:
Peng SHEN
;
Youbei QIAO
;
Rui MA
;
Rui ZHANG
;
Wenrui MA
;
Shaofeng ZHANG
;
Jiang WU
- Publication Type:Journal Article
- Keywords:
Polylactic acid(PLA);
Chitosan(CS);
Recombinant human bone morphogenetic protein-2(rhBMP-2);
Stromal cell-derived factor-1(SDF-1);
Controlled release
- From:
Journal of Practical Stomatology
2016;32(2):161-166
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct double-layered controlled release system containing SDF-1 and rhBMP-2 molecules and to study the release profile of the system in vitro.Methods:The polylactic acid/chitosan(PLA/CS)nanoparticles were prepared with “emulsification-solution evaporation method”,the preparation parameters were determined by orthogonal test design.The particle size was observed by nanoparticle size analyzer,the morphology of the nanoparticles was observed with electron microscope.Then rhBMP-2 and SDF-1 were loaded into the nanoparticles in the process of emulsification,the loading efficiency and encapsulation efficiency were calculated and in vitro release was observed.Results:The double-layer nanoparticles showed spherical geometry,smooth surface and complete separation. The average particle size of the nanoparticles was (542.33 ±14.38)nm;The drug loading and incorporation efficiency of rhBMP-2 were (82.41 ±1.05)% and (24.67 ±0.43)ng/mg,those of rhBMP-2 were (75.58 ±0.84)% and (22.63 ±0.41)ng/mg,respectively. The release time of the drug from the system sustained over at least 30 days,the release profile of both drugs showed “biphasic release”. The cumulative release rate of SDF-1 and rhBMP-2 was 72.85% and 91.01% in 30 days respectively.Conclusion:The SDF-1 and rh-BMP-2 loaded PLA/CS nanoparticles have excellent morphology,high entrapment and good sustained-release in vitro.
