Effect of miR-199a/b-3p on cell proliferation of TNBC cells
10.3969/j.issn.1000-484X.2016.04.006
- VernacularTitle:MicroRNA-199 a／b-3 p抑制三阴乳腺癌细胞增殖机制研究
- Author:
Zihang WANG
;
Zhicheng WANG
;
Jiaxing ZHANG
;
Yunda FAN
;
Jinsong KANG
;
Xuguang MI
- Publication Type:Journal Article
- Keywords:
TNBC;
microRNA-199a/b-3p;
Proliferation
- From:
Chinese Journal of Immunology
2016;32(4):480-482
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the inhibiting mechanism of microRNA-199a/b-3p(miR-199)on cell proliferation of triple negative breast cancer(TNBC)cells.Methods:Expression of miR199 in BT549 and MDA-MB-231 cells transfected with miR-199a/b-3p was detected by qRT-PCR.The proliferation of BT549 and MDA-MB-231 cells transfected with miR-199 were analysed by MTT as-say.Cell cycle of TNBC cells transfected with miR-199 was detected by Flow Cytometry assay.Results:MiR-199a/b-3p could suppress the proliferation of BT549 and MDA-MB-231 cells.Comparing with normal control,the proliferation rate were up to(41.02±2.34)%and(28.42 ±6.70 )%,and the cell cycle were arrest at G 1 phase.Conclusion: MiR-199a/b-3p could suppress the proliferation of TNBC,and may be a promising anti-cancer drug for TNBC.
