Enhancement of γδT cells proliferation and cytotoxicity by Hyperoside
10.3969/j.issn.1000-484X.2016.04.016
- VernacularTitle:金丝桃苷增强人γδT细胞增殖及杀伤功能研究
- Author:
Ying LI
;
Yu ZHOU
;
Leiqing SUN
;
Zhonghai ZHOU
;
Xiaoting Lü
;
Ming XU
;
Yi LI
;
Junquan LIU
- Publication Type:Journal Article
- Keywords:
Hyperoside;
γδT cells;
DU-145 cell;
Cytotoxicity
- From:
Chinese Journal of Immunology
2016;32(4):524-527
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the anti-tumor effect of Hyperoside.Methods: Human γδT cells were amplified by isopentenyl pyrophosphate from peripheral blood cells.The proliferation capacity of γδT cells was measured with CCK-8 assay after treated with different concentrations of Hyperoside.Cytotoxicity of γδT cells was detected with LDH assay , and the expression of granzyme,perforin CD107a and IFN-γonγδT cells were measured by flow cytometry before and after treatment.Results: Hyperoside could significantly stimulate the proliferation of γδT cells at the concentration of 3.13-12.5 μg/ml.Cytotoxicity and expression of granzyme,perforin and IFN-γofγδT cells were increased after treatment.Conclusion:Hyperoside could enhance cytotoxicity of humanγδT cells through up-regulation of granzyme ,perforin CD107 a and IFN-γexpression.
