Bioinformatics analysis of VP1 protein of coxsackievirus A6
10.3969/j.issn.1000-484X.2016.04.019
- VernacularTitle:柯萨奇病毒A6型VP1蛋白的生物信息学分析
- Author:
Hongbo LIU
;
Guangfei YANG
;
Weilin OU
;
Guanxin SHEN
- Publication Type:Journal Article
- Keywords:
Coxsackievirus A6;
VP1 protein;
Bioinformatics;
B-cell epitope;
Physicochemical property
- From:
Chinese Journal of Immunology
2016;32(4):536-541
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To predict the basic physicochemical properties ,structure and function ,and linear B-cell epitopes of the capsid protein VP1 of coxsackievirus A6(CVA6).Methods: The amino acid sequence of the CVA6 VP1 was analyzed using Bioedit software and various online tools including SubLoc ,TargetP and the others from ExPASy Bioinformatics Resource Portal.Results: The CVA6 VP1 protein was a hydrophilic protein with a relative molecular weight of 33.6 kD and an isoelectric point of 7.92.This protein containsed 24 phosphorylation sites , but no signal peptide , transmembrane domains and possible fatty acylation sites.Its secondary structure was characterized by the richest random coils , and 48.52 percent of its amino acid residues exposed at the solution inter-face.Epitope prediction by Bepipred showed a number of potential B cell epitopes in the protein ,the highest antigenicity index among them located in the region of amino acids residue 155-165.Conclusion:The basic physicochemical properties ,structure and function characteristics ,and potential linear B-cell epitopes of CVA 6 VP1 were successfully predicted , which laid foundations for the further study on the protein and the preparation of vaccines and immunological diagnostic reagents for CVA 6 infection.
