Changes and correlation of lymphocyte subsets and serum levels of IgE in children with allergic rhinitis
10.3969/j.issn.1000-484X.2016.04.022
- VernacularTitle:过敏性鼻炎患儿淋巴细胞亚群、血清IgE水平的变化及其相关性
- Author:
Wenzhong XU
;
Chunyan LIU
- Publication Type:Journal Article
- Keywords:
Lymphocyte subsets;
IL-4;
IL-5;
Interferon γ;
IgE;
Allergic rhinitis;
Flow cytometry
- From:
Chinese Journal of Immunology
2016;32(4):550-552
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the changes of lymphocyte subsets and the serum levels of IgE in the children with allergic rhinitis,and to analyze the relationship between lymphocyte subsets and IgE.Methods:CD3+,CD4+,CD8+CD19+,CD4+CD25+,CD16+56+lymphocyte subsets were measured by flow cytometry ,IFN-γ,IL-4,IL-5 of serum were detected by enzyme linked immunosorbent assay(ELISA)and the serum level of total IgE was detected by immuno turbidimetric assays in 86 cases of children with allergic rhinitis and 76 cases of healthy children.Results:CD19+B lymphocyte subsets ,the ratio of CD4+and CD8+,IL-4 ,IL-5 and the serum levels of IgE were increased in the children with allergic rhinitis groups as compared with healthy children , the difference has statistical significance(P<0.05);CD3+,CD4+,CD8+,CD4+CD25+,CD16+56+lymphocyte subsets and IFN-γwere lower than the control group ( P<0.05 );there was a correlation between B lymphocyte subsets and IgE in the children with allergic rhinitis.Co nclusion:Lymphocyte dysfunction and Th1/Th2 ratio imbalance may be related to the pathogenesis of allergic rhinitis ,and IgE,IFN-γ,IL-4 and IL-5 play important roles in children with allergic rhinitis.
