Determination of Ferulic Acid and Caffeic Acid in Atractylodes Macrocephala Koidz from Different Habitats by HPLC
- VernacularTitle:HPLC法同时测定不同产地生白术中阿魏酸和咖啡酸的含量
- Author:
Deke ZHANG
;
Chuanjiang MA
- Publication Type:Journal Article
- Keywords:
HPLC;
Atractylodes macrocephala Koidz;
Ferulic acid;
Caffeic acid
- From:
China Pharmacist
2016;19(3):594-595
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop an HPLC determination method for ferulic acid and caffeic acid in Atractylodes macrocephala Koidz fromdifferentorigins.Methods:ThecolumnwasAgilent-C18(150mm×4.6mm,5μm),themobilephasewas0.2% acetonitrile-aceto-nitrile(70 ∶30),the UV detection was performed at 320 nm,the flow rate was 1. 0 ml·min-1, and the drift tube temperature was set at 35℃. Results:The calibration curve was linear within the range of 0. 040-0. 500μg for ferulic acid(r=0. 999 8)and 0. 060-0. 750 μg for caffeic acid(r=0.999 8). The average recovery of ferulic acid and caffeic acid was 98.10%(RSD =1.08%)and 98.66%(RSD =0. 61%)(n=6), respectively. Conclusion: The method is simple and accurate. It is suitable for the determination of ferulic acid and caffeic acid in Atractylodes macrocephala Koidz.
