Determination of β-Eudesmol in Cortex Magnoliae Officinalis by Capillary Gas Chromatography
- VernacularTitle:毛细管气相色谱法测定厚朴中β-桉叶醇的含量
- Author:
Zhangjin CHEN
;
Huali YU
;
Juhua MAO
;
Lei FAN
;
Jiana JI
- Publication Type:Journal Article
- Keywords:
Cortex Magnoliae Officinalis;
β-Eudesmol;
Assay;
GC
- From:
China Pharmacist
2016;19(3):602-604
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a quantitative determination method for β-eudesmol in Cortex Magnoliae Officinalis by GC. Methods:β-Phenethanol was used as the internal standard substance;the column was a Zebron ZB-WAX capillary column ( 60 m × 320 μm,0. 5μm) with the column temperature of 200℃;the detector was FID and the vaporizer temperature was 250℃; the carrier gas was nitrogen with the flow rate of 1. 3 ml · min-1 and the split ratio was 4 ∶1. Results: The linear range of β-eudesmol was 0. 015 1-0. 271 2 mg·ml-1(r=0. 999 8);the average recovery was 99. 28%(RSD =1. 17%, n=6). Conclusion:The method is simple and accurate with good reproducibility, which can be used for the quality control of medicinal materials and decoction pieces of Cortex Magnoliae Officinalis.
