Qualitative Identification and Quantitative Analysis of Paeoniae Radix Rubra in Fufang Fuqing Lotion
- VernacularTitle:复方肤清洗剂中赤芍的定性鉴别和定量分析
- Author:
Hui XIONG
;
Jinmin SHI
;
Can ZHOU
;
Qingwei ZOU
;
Xiaoyan ZHANG
- Publication Type:Journal Article
- Keywords:
Fufang Fuqing lotion;
Paeoniae radix Rubra;
Paeoniflorinl;
HPLC
- From:
China Pharmacist
2016;19(3):616-617
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the qualitative identification and quantitative analysis of Paeoniae radix Rubra in Fufang Fuqing lotion. Methods:TLC was used to identify Paeoniae radix Rubra. The content of paeoniflorinl was determined by HPLC. The mobile phase was acetonitrile-0. 01% phosphonic acid (13 ∶87), and the detection wavelength was 230 nm,the flow rate was 1. 0ml·min-1, the column temperature was 40℃,and the sample size was 10μl. Results:The results of TLC showed that the relevant spots were clear without any interference from the negative sample. The calibration curve of paeoniflorinl was linear within the range of 0. 070-4. 500 μg (r=1. 000 0). The average recovery was 98. 36% with RSD of 2. 73%(n=6). Conclusion:The methods are accurate and quick in the qualitative identification and quantitative assay of the preparation, which can be used for the quality control of Fufang Fuqing lo-tion.
