Influence of three types of scaffolds on biological behavior of stem cells from human exfoliated deciduous teeth
10.3969/j.issn.1001-3733.2016.02.017
- VernacularTitle:体外观察三种支架材料对人乳牙牙髓干细胞生物学行为的影响
- Author:
Ling YU
;
Yang LIU
;
Yuanyuan ZHANG
;
Na XIE
;
Fei LIU
;
Qingyu GUO
- Publication Type:Journal Article
- Keywords:
Tissue engineering;
Stem cells from human exfoliated deciduous teeth(SHEDs);
Scaffold
- From:
Journal of Practical Stomatology
2016;32(2):235-238
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the adhesion,proliferation and differentiation of the stem cells from human exfoliated deciduous teeth(SHEDs)on 3 different types of hydroxyapatite(HA)composite scaffold materials.Methods:Pulp cells from human exfoliated de-ciduous teeth were harvested from impacted deciduous teeth by enzyme digestion,expanded and cultured.Cells were verified by immuno-histochemical methods and in vitro differentiation test.Then the cells were cultured on HA/beta tricalcium phosphate (HA/TCP),HA/collagen (HA/COL)and HA/poly-ethylene propylene lactide (HA/PLGA)scaffold respectively.Adhesion rate was examined at hour 4,6,8 and 10 of culture,proliferation was observed by MTT assay on day 1,4,7 and 10 of culture,respectively.The osteogenic dif-ferentiation was studied by alkaline phosphatase(ALP)test,Von Kossa staining and calcium content measur.Results:The attachment of SHEDs was significantly lower on the HA/COL than on the other 2 scaffolds(P <0.05).The ALP activity,mineralization and calci-um content were the highest on HA/PLGA,and the last on HA/COL(P <0.05).Conclusion:HA/PLGA scaffold is more effective in the promotion of the proliferation,attachment and differentiation of SHEDs than HA/TCP and HA/COL scaffolds.
