The effects of microgroove surface form of titanium on the adhesion and cell cycle progression of human gingival fibroblasts
10.3969/j.issn.1001-3733.2016.01.019
- VernacularTitle:微沟槽形貌对人牙龈成纤维细胞黏附及细胞周期推进影响的研究
- Author:
Yingzhen LAI
;
Shan LIN
;
Jiang CHEN
- Publication Type:Journal Article
- Keywords:
Soft tissue;
Implant;
Microgroove;
Human gingival fibroblasts;
Contact guidance;
Cell cycle
- From:
Journal of Practical Stomatology
2016;32(1):89-95
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of microgroove surfaces of titanium on the adhesion and cell cycle progression of human gingival fibroblasts(HGFs).Methods:Microgroove titanium surfaces were fabricated on silicon plate by photolithography with parallel grooves:1 5,30 and 60 μm in width and 5 μm and 1 0 μm in depth,the groups were denoted as T1 5 /5,T1 5 /1 0,T30 /5,T30 /1 0, T60 /5 and T60 /1 0,respectively.Smooth titanium surfaces (T0)were used as the controls.Surface topography were observed by ES-EM.HGFs were cultured on the microgroove surfaces.Morphology of “contact guidance”was observed by immunofluorescence tech-nique.Cell cycle distribution was analyzed by Flow cytometry.Results:HGFs on the microgroove surfaces had “contact guidance”par-allel to the microgrooves,whereas the cells on T0 were oriented randomly.T60 /1 0 group had the highest percentage of S phase cells, followed by T30 group and T1 5 group,but still higher than that in the control group.In groups with higher groove width (T60 group and T30 group),the increase of groove depth benefited the increase of S phase percentage,while in T1 5 group,the increase of groove width decreased the S phase percentage.Conclusion:Surfaces of microgrooves with different dimensions achieved “contact guidance”for the cultured HGFs.The surfaces with increasing groove width and depth benefit the cell cycle progression.
