Effect of recombinant hIL-10 on lymphocytes and IL-17A of an AA rat model
10.3969/j.issn.1000-484X.2016.04.005
- VernacularTitle:重组hIL-10对AA大鼠T淋巴细胞及IL-17 A的影响研究
- Author:
Lianfeng DU
;
Wanbang SUN
;
Limin DING
;
Yanli TANG
- Publication Type:Journal Article
- Keywords:
Recombinant hIL-10;
Adjuvant arthritis;
T cell subsets;
Interleukin-17A
- From:
Chinese Journal of Immunology
2016;32(4):476-479
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Adjuvant arthritis(AA)rats interfered with recombinant hIL-10,methotrexate(MTX)separately,we detected the changes of T cell subsets in rat′s blood and IL-17 A in rat′s serum.Methods:AA rats interfered with recombinant hIL-10, MTX and IL-10 plus MTX.The changes of the CD4+and CD8+T cell subsets were detected by flow cytometry;the Levels of IL-17A in serum of rats were measured by ELISA method.Results:Compare to normal control group ,the CD4+T cell total quantity and CD4+/CD8+T cell Proportion was significantly decreased , there were significant differences between the treatment group and the untreated group(P<0.05 ), the IL-17A of treatment group was significantly decreased , there was a significant differences ( P<0.05 ).The combination group had significant difference compared with IL-10 group.Conclusion:Recombinant hIL-10 can down-regulate the blood of AA rats in the number of CD4 +T cells and CD4+/CD8+ratio,reduce serum levels of IL-17A.The combination group compared with the IL-10 group effect on serum IL-17A more significantly,the results displayed recombinant hIL-10 plus MTX in the treatment of rheumatoid arthritis can better play the role.
