Normal group and artificial simulation of cold environment proteomics of lung tissue of rats variance analysis
10.3969/j.issn.1000-484X.2016.04.008
- VernacularTitle:正常组与人工模拟寒环境组大鼠肺组织蛋白组学差异分析
- Author:
Ping WANG
;
Fangfang LIU
;
Junlian LI
;
Yanping MA
;
Xiaowei ZHANG
;
Yanyan LI
;
Weijun ZHANG
;
Gongding TAO
- Publication Type:Journal Article
- Keywords:
Cold environment;
Lung tissues;
Proteomics;
Variance analysis
- From:
Chinese Journal of Immunology
2016;32(4):486-489
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyse and discuss the variance and mass spectrometry of proteomics of rats 'lung tissues between groups of normal and simulated cold environments.Methods:A total of 20 SD rats were randomly divided into two groups of normal and simulated cold environments with 10 rats in each group.The temperature of normal environment group was controlled in 21℃ with a fluctuation of 2℃lasting 24 h per day.In contrast, the temperature of simulated cold environment group was controlled in 6℃ with a fluctuation of 2℃lasting 4 h per day while the temperature and humidity for the rest of time were exactly the same as the situation in normal environment group.The preoperative fasting , water-deprivation and weighting were adopted 12 h before anaesthesia to both groups after the continuing 7-day experimentation.Left lung tissues of each rat in both groups were removed by the amount of 10 mg, whereas three random samples from each group were tested by experiments of protein extraction , bidirectional electrophoresis , image analysis and mass spectrometry identification.Results: Given the proteomics analysis of two differential sets , it is indicated that in simulated cold environment group , among nine sorts of protein , four sorts perform rising while five falling compared to that of normal group.Conclusion:There exists a correlation between protein and cold environment based on the results of proteomic analysis .
