Effect and mechanism of transcription factor Foxp3 on proliferation and cell cycle of human lung adenocarcinoma cell A549
10.3969/j.issn.1000-484X.2016.04.009
- VernacularTitle:转录因子Foxp3对人肺腺癌细胞A549增殖周期的影响及机制研究
- Author:
Yaqing LIU
;
Bo ZHAO
;
Yadong LUO
;
Yinan LI
;
Wei YANG
- Publication Type:Journal Article
- Keywords:
Foxp3;
CCND1;
Lung adenocarcinoma;
Proliferation;
Cell cycle
- From:
Chinese Journal of Immunology
2016;32(4):490-494
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and mechanism of transcription factor Foxp 3 on the proliferation and cell cycle of human lung adenocarcinoma cell line A 549.Methods:We knocked down the expression of Foxp 3 using siRNA.Foxp3 inhibition was detected by RT-PCR.Cell proliferation was detected by MTT.Cell cycle of A549 cells were detected by flow cytometry after the transfection of siRNA.Cell cycle-related checkpoint genes were filtered by RT-PCR.The regulation of Foxp3 on cell cycle-related checkpoint genes were detected by immunofluorescence and dual -luciferase reporter assay system.Results: The proliferation of A549 cells were inhibited after silencing Foxp3,and A549 cells were arrested in G0/G1 cycle.G1/S cycle checkpoint gene CCND1 was down regulated.Mechanism research show that Foxp 3 can regulate the expression of CCND 1 directly.Conclusion: Foxp3 can promote the proliferation of A549 cell line by up regulating G 1/S cycle checkpoint gene CCND 1.This provides a new target for the therapeutic targets of lung adenocarcinoma.
