Study of Qingluo Tongbi Compound treating rheumatoid arthritis based on miRNA network
10.3969/j.issn.1000-484X.2016.04.010
- VernacularTitle:清络通痹方调控miRNA网络干预类风湿关节炎的研究
- Author:
Yamei ZHU
;
Lingling ZHOU
;
Xiaowu PENG
;
Shan GENG
;
Xueping ZHOU
- Publication Type:Journal Article
- Keywords:
microRNA;
CIA;
Qingluo Tongbi Compound ( QLT)
- From:
Chinese Journal of Immunology
2016;32(4):495-499
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mechanism of Qingluo Tongbi Compound (QLT) treating rheumatoid arthritis(RA)by observing miRNA Network of QLT on collagen-induced arthritis ( CIA) mice.Methods:The model of RA was induced by collagenⅡin DBA/1 mice and randomly divided into control group , CIA group, QLT group.Differently expressed miRNAs were detected by miRCURYTM LNA Array.Real-time PCR was applied to verify the reliability of miRNA array.Results:The bioinformatics software and database were applied to predict and analyze target genes.MiRNA array results showed that 221 miRNAs changed in CIA group compared with the control group ,and 169 miRNAs changed in QLT group compared with CIA group.And the results of real-time PCR were consistent with the array.Compared with the control group ,miR-143 was significantly reduced in CIA group ,intervention of QLT obviously upregulated the expression of miR-143.The target genes of miR-143 were significantly stored in VEGF , T cell receptor, MAPK,signaling pathway.Conclusion: Multiple abnormal expression of miRNAs involved in the pathological process of CIA.QLT affected the expression of various miRNAs ,which might be related to immunity ,inflammation,pain pathological process of RA and miR-143 could be a potential target in the treatment.
