Screening tumor vaccine adjuvants from four kinds of Chinese herbal monomer in vitro
10.3969/j.issn.1000-484X.2016.04.012
- VernacularTitle:四种中药单体作为肿瘤细胞疫苗佐剂的体外筛选研究
- Author:
Lina ZHANG
;
Shuang WANG
;
Zeyuan LIU
;
Li ZHANG
;
Ling LI
;
Ming HU
;
Bin WANG
- Publication Type:Journal Article
- Keywords:
Chinese herbal monomers;
Procyanidin;
Baicalein;
Immunological effects;
Screening;
Adjuvant
- From:
Chinese Journal of Immunology
2016;32(4):504-508
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect immunological effects of procyanidin ,gastrodin,baicalein and apigenin on antigen presenting cells of RAW264.7 and DC2.4 and search for new tumor vaccine adjuvant.Methods: After different concentrations of four kinds of Chinese herbal monomer and LPS were used to stimulate RAW 264.7 or DC2.4 cells for 48 hours,cells were obtained and stained with CD80,CD86,MHCⅠand MHCⅡ antibody.Then protein expression levels of CD80,CD86,MHCⅠ and MHCⅡ on RAW264.7 and DC2.4 cells were analyzed by flow cytometry.Results: Results showed that after 48 hours stimulated with different concentrations of four kinds of Chinese herbal monomer on RAW 264.7 or DC2.4 cells,the expression levels of CD80,CD86,MHCⅠ,MHCⅡ were all up-regulated in comparison with control , procyanidin and baicalein exhibited stronger immunological stimulating activity on a cellular level.Conclusion:Procyanidin and baicalein were found having potential application value in clinical treatment as tumor vaccine adju -vants.
