Interaction between MMS2 and P53 on proliferation and apoptosis of colon cancer cells

VernacularTitle:MMS2和P53基因对结肠癌细胞增殖与凋亡的影响
Author: Linyuan MA ; Yu SUI ; Lu MA ; Xin LI ; Yuanjie LI ; Fang XU
Publication Type:Journal Article
Keywords: MMS2; P53; RNA-interference; Human colon cancer cells; Apoptosis
From: Chinese Journal of Immunology 2016;32(4):513-518
CountryChina
Language:Chinese
Abstract: Objective:To explore the regulatory effects of proliferation and apoptosis on THC-8307 by MMS2 siRNA and P53 siRNA.Methods:We experimentally suppressed the MMS2 and P53 expression in human colon cancer cells by the interference RNA technology ( RNAi) as monitored by Real-time qRT-PCR and Western blot.THC-8307 cells that express rate significantly reduced were collected as case group , while using untreated cells as the blank control group , and mock-treated cells as the negative control group.After separately interfering the target genes in each group ,test the relationship and expression level of the two genes.Utilizing flow cytometry techniques to test cells proliferation and apoptosis rate of each group.Results: Compared to the control group , colon cancer cells in which MMS2 and P53 were silenced displayed significant increase of P53,MMS2 mRNA and protein levels(P<0.05).MMS2-depleted cells displayed increase in apoptosis rates ,for both early and later stages ( P<0.05 ).Conclusion: MMS2 and P53 negatively regulate each other in colon cancer cells proliferation and apoptosis .