Methodological evaluation of rhCNB in long-tailed macaque sera detected by Enzyme-linked Immunosorbent Assay(ELISA)
10.3969/j.issn.1000-484X.2016.04.017
- VernacularTitle:ELISA法检测食蟹猴血浆中rhCNB多肽的方法学评价
- Author:
Jiping SHAO
;
Caiyun ZHANG
;
Buwei HU
;
Xueli XIE
;
Shuhong TIAN
;
Richao WANG
;
Daolong HUANG
;
Jian FU
- Publication Type:Journal Article
- Keywords:
Enzyme linked immunosorbent assay (ELISA);
Long-tailed macaque;
Recombinant human calcineurin B subunit ( rhCNB);
Methodological evaluation
- From:
Chinese Journal of Immunology
2016;32(4):528-531
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To validate an enzyme linked immunosorbent assay (ELISA) method for the quantification of rhCNB in long-tailed macaque sera.Methods: The linear,sensitivity,accuracy,precision and recovery were determined using ELISA.Results:The present ELISA method had high linearity within 0.195 ng/ml-12.5 ng/ml,the working curve of rhCNB was Y=15.1X-0.26, R2=0.996 8 , the method showed good sensitivity of 0.195 ng/ml, the accuracy were in the range of 91.9%-108.8%, and the Coefficient of variation ( CV) for inter-assay were 3.55%,1.39%and 4.71%,the intra-assay were 1.59%,3.2%and 3.8%,all less than 10%, the recoveries were in the range of 88.5% -108.3%, <110% .Thus the method was coincidence with requirement.Conclusion:Double antibody sandwich ELISA assay of rhCNB in long-tailed macaque sera has good sensitivity ,accuracy, precision and recovery and it can be used to measure rhCNB concentration in biological samples .
