Determination of Dracohodin in Qilisan Gel by HPLC
- VernacularTitle:七厘散凝胶剂中血竭素含量的HPLC测定
- Author:
Shuyun JIANG
;
Hong LI
;
Mengliang JIANG
;
Qun HE
;
Xiaohuang JIANG
;
Jinwen GE
- Publication Type:Journal Article
- Keywords:
QiliSan gel;
Dracohodin;
HPLC;
Quality control
- From:
China Pharmacist
2016;19(3):589-591
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for determining the content of dracorhodin as an indicator in the quality control with methodological studies to provide basis for the preparation process study of Qilisan gel. Methods: An HPLC method was used with a DiamonsilR C18 (250 mm ×4. 6 mm, 5 μm) column, the detection wavelength was set at 440 nm and the column temperature was 30 ℃. Acetonitrile-0. 05 mol·L-1 sodium dihydrogen phosphate solution (45 ∶ 55) was used as the mobile phase, and the flow rate was l. 0 ml·min-1 . Results: The content of dracorhodin was in a good linear relationship (r =0. 999 6) within the range of 1. 632-64. 250 μg·ml-1 , and the average recovery was 99. 02%(RSD =0. 77%, n =6). The number of theoretical plates was calculated as 7 100 according to the dracorhodin perchlorate peak without any interference from the negative sample. Conclusion: The method is objective, accurate and sensitive with high reliability, easy operation and fast process. The measurement results can be used as the quality control basis for the preparation process study of Qilisan gel.
