Effect of Dimethyl Dicarboxylate Biphenyl (DDB) on the Proliferation, Apoptosis and PPARγExpression of Hepatic Stellate Cells
- VernacularTitle:联苯双酯对大鼠肝星状细胞增殖和凋亡及PPARγ表达的影响
- Author:
Danyun WEI
;
Hong ZHANG
;
Ying ZHANG
;
Rui PEN
- Publication Type:Journal Article
- Keywords:
Dimethyl dicarboxylate biphenyl;
Hepatic stellate cells;
PPARγ;
Proliferation;
Apoptosis
- From:
China Pharmacist
2016;19(3):413-416
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of dimethyl dicarboxylate biphenyl ( DDB) on the proliferation, apoptosis and PPARγ expression of hepatic stellate cells. Methods: HSC-T6 cells were cultured in 96-well plates and 6-well plates, and after the 24-hour drug treatment, the influence of DDB on the proliferation and apoptosis of HSC-T6 were detected respectively by CCK-8 kit and flow cytometry. Quantitative real-time-PCR ( Q-PCR) and Western blotting were adopted to determine the effect of DDB on the PPARγmRNA level and the protein expression in HSC-T6 cells. Results:DDB obviously inhibited the proliferation of HSC-T6 (P<0. 05) and significantly promoted the apoptosis of HSC-T6 (P<0. 05) at the experimental concentration (8-64 μmol·L-1) when compared with the control group (0 μmol·L-1). The expression of PPARγ in drug-treated HSC-T6 was notably improved. Conclusion: DDB can inhibit the proliferation and promote the apoptosis of HSC-T6 cells by up-regulating the expression of PPARγ.
