Effect of Different Purification Technology on Physical and Chemical Properties of Water Extract of Ophio-pogonin
- VernacularTitle:不同纯化技术对麦冬皂苷水提液物理化学性质的影响
- Author:
Yanni LIANG
;
Zhishu TANG
;
Ying TIAN
;
Chuan GU
;
Shijun LIU
;
Hongbo LIU
- Publication Type:Journal Article
- Keywords:
Ophiopogonin;
Water extract;
Physical and chemical properties;
Purification technology
- From:
China Pharmacist
2016;19(3):430-433
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of different separation and purification technology on the physical and chemical proper-ties of water extract of ophiopogonin. Methods:The water extraction process of total ophiopogon saponins was optimized by an orthogo-nal test. The macroporous resin adsorption and membrane separation technology were adopted to purify the ophiopogonin water extract. The physical and chemical parameters, such as electrical conductivity, pH value, viscosity and turbidity, and the contents of total sap-onins, proteins, tannins and polysaccharides were determined. Results:The optimum water extraction technology of total saponins was as follows:6-fold amount of water was added, boiling 3 times with 90 min for each time. The electrical conductivity, pH value and vis-cosity of different purified liquid of total saponins had no significant differences, while the contents of total saponins and the three kinds of macromolecules showed significant differences. Conclusion: The content of macromolecules can be used as the reference index of purification process of total saponins water extract. Compared with macroporous resin separation, membrane separation technology is more suitable for the separation and purification of total saponins water extract.
