Determination of chlorogenic acid and galuteolin in Jinmei-Qingshu granule by HPLC
10.3760/cma.j.issn.1673-4246.2016.02.016
- VernacularTitle:HPLC测定金梅清暑颗粒中绿原酸与木犀草苷的含量
- Author:
Jie LIN
;
Zhihao LI
- Publication Type:Journal Article
- Keywords:
Chlorogenic acid;
Galuteolin;
Chromatography,high pressure liquid;
Jinmei-Qingshu granule
- From:
International Journal of Traditional Chinese Medicine
2016;(2):159-162
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the HPLC method for the determination of chlorogenic acid and luteoloside in Jinmei-Qingshu granule. Methods The DIONEX C18 (250 mm×4.6 mm, 5 μm) column was used, the mobile phase consisted of acetonitrile:0.4%H3PO4, gradient elution, the flow rate was 0.8 ml/min, the column temperature was 30 ℃, and the detecting wavelength was at 330 nm. Results The cablibration curve was linear within a range of 14.10-282.00 μg and 0.60-12.00 μg, the average recovery of this method was 98.87%, 97.75% and the RSD were 1.44%, 1.52%, respectively. Conclusions The method was highly sensitivity, accurate, repeatable and with high specificity. It can be an important method to control the standard of Jinmei-Qingshu granule.
