Detection and identiifcation of serum biomarker for ganglioneuroblastoma in children

VernacularTitle:儿童节细胞神经母细胞瘤血清特异性蛋白的筛选与鉴定
Author: Yang YANG ; Jiaxiang WANG ; Fei GUO ; Da ZHANG ; Jia JIA
Publication Type:Journal Article
Keywords: ganglioneuroblastoma; proteomics; apolipoprotein C-Ⅲ; child
From: Journal of Clinical Pediatrics 2016;(2):124-127
CountryChina
Language:Chinese
Abstract: Objective To build a more perfect serum protein ifngerprint models for early diagnosis of ganglioneuroblas-toma (GNB) in children. Methods Thirty children with GNB and 30 normal control children were recruited. Serum samples were collected. Nonspeciifc serum protein was detected and studied by MB-WCX processing, SELDI-TOF-MS mass spectrom-etry system and MALDI-TOF/TOF platform. Results Through the SELDL-TOX-MS processing, a peak at 5920 m/z protein markers, and the expression of the markers was high in GNB children (6180.6±2328), compared with normal control children (419.1±493.3), the difference was statistically signiifcant (P<0.05);MALDI-TOF/TOF platform showed that the protein with a peak at 5920 m/z is identiifed as ApoC-Ⅲ. Conclusions m/z peak of 5920 protein is suggested as speciifc biomarker of GNB in children, can provide signiifcant reference for early diagnosis of ganglioneuroblastoma, and prognostic monitoring.