DTBNP and DTDP increase glucose-stimulated insulin secretion in INS-1 cell
10.3969/j.issn.1006-5725.2016.06.005
- VernacularTitle:DTBNP、DTDP促进INS-1细胞葡萄糖刺激胰岛素分泌
- Author:
Minglin PAN
;
Saimaiti JULAITI
;
Tian LIU
;
Yanyan GUO
- Publication Type:Journal Article
- Keywords:
Glucose-stimulated insulin secretion;
INS-1 cell;
Sulfydra lredox agents
- From:
The Journal of Practical Medicine
2016;32(6):883-886
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of sulfydral redox agent in the modulation of insulin secretion and the potential mechanism. Methods Insulin secretion was evaluated in INS-1 cells after treatment with different concentrations of glucose and sulfydral redox agents by a standard insulin radio immunoassay. Results Glucose concentration-dependently potentiates insulin secretion was observed in INS-1 cells. DTBNP and DTDP could not only significantly increase glucose-stimulated insulin secretion (GSIS), but also increase insulin secretion in nifedipine-pretreated cells, which could be abrogated by DTT. Importantly, pharmacological ablation of L-type calcium channels by nifedipine and/or ablation of K ATP channelby diazoxide both could potentiate glucose-induced insulin secretory. Conclusions Sulfydral redox agent could regulates GSIS. DTBNP and DTDP may increase insulin secretion via regulating the activities of KATP, L-type CaV channel and IP3 receptor.
