Establishing a large double-foot bird emu model of femoral head necrosis
10.3969/j.issn.2095-4344.2016.05.001
- VernacularTitle:构建双足类大型鸟类鸸鹋股骨头坏死模型
- Author:
Chuntao QU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(5):611-615
- CountryChina
- Language:Chinese
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Abstract:
BACKGROUND:There are many studies on the establishment of animal models of femoral head necrosis, but it is difficult to simulate an ideal animal model of femoral head necrosis, especialy colapse models. OBJECTIVE: To establish femoral head necrosis model of double-foot large bird emu so as to simulate femoral head necrosis in human. METHODS: A total of 30 adult emus were frozen in liquid nitrogen and received radiofrequency heating alternately to simulate femoral head necrosis models. After 3 cycles of freezing, local frozen in liquid nitrogen and method of radiofrequency heating was alternated for closed loop liquid nitrogen freezing injury and radiofrequency heating injury. At 6, 12 and 16 weeks after model establishment, gross observation, X-ray, histology examination and MRI were conducted. Effects of model induction of avascular necrosis of the femoral head were evaluated. RESULTS AND CONCLUSION: After liquid nitrogen cold-hot alternating method, emu at 12-18 weeks gradualy suffered from femoral head necrosis signs and imaging findings. X-ray films, histological examination and MRI examination showed the avascular necrosis of femoral head was consistent with the pathological process of human femoral head necrosis, and the model had good repeatability. These results indicate that the use of liquid nitrogen cold-hot alternating method has successfuly established the model of femoral head necrosis, with a goodcolapse rate, and can be used for mechanism research and treatment evaluation of osteonecrosis.
