The application of manganese-enhanced MRI in stereotaxic techniques of rat visual nuclei
10.3969/j.issn.1002-1671.2016.02.034
- VernacularTitle:锰离子增强 MRI 在大鼠视觉中枢核团立体定位中的运用
- Author:
Jun YANG
;
Qinqing LI
;
Yingying DING
;
Guangjun YANG
;
Yu XIE
;
Chan ZHU
;
Chengde LIAO
;
Zhuolin LI
- Publication Type:Journal Article
- Keywords:
manganese;
magnetic resonance imaging;
visual nuclei;
stereotaxic techniques
- From:
Journal of Practical Radiology
2016;(2):295-298
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the value of manganese-enhanced MRI in locating the rat visual nuclei.Methods The visual nuclei of thirty-six rats were located by 3 different ways including individual MEMRI locating (group A,n= 1 6),anatomical atlas locating (group B,n=1 6)and direct puncture by using the data obtained in MEMRI (group C,n=4).After unilateral intra-vitreal injection of MnCL2 (30 mmol/L×3 μL)in group A,the brain MRI was performed 24 h later.The location coordinate of lateral geniculate nucleus (LGN) and superior colliculus (SC)were recorded individually.The nuclei injections (3% fluorogold solution,1 μL)were performed by using different location coordinate in groups A and B.The rat’s retinas were examined under fluorescence microscope 5 days later,and the results were compared between the two groups.After brain nucleus puncture injection (30 mmol/L MnCL2 solution,0.5 μL),MRI was performed 1 h later in group C.Results The success rate was 93.8% (1 5/1 6)in group A,and 65.5% (10/1 6)in group B.The difference between groups was statistically significant (P<0.05).All the injection locations of C group were agreed with atlas.Conclusion MEMRI in the visual nucleus stereotactic can improve the accuracy of location.
