Changes in vimentin and vascular endothelial growth factor expression in a rat model of thin endometrium established by 95% ethanol
10.3969/j.issn.2095-4344.2016.05.019
- VernacularTitle:体积分数95%乙醇建立薄型子宫模型大鼠子宫内膜中波蛋白和血管内皮生长因子的变化
- Author:
Yanping XU
;
Guifeng ZHAO
;
Jianing MIAO
;
Jichun TAN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(5):718-722
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Ethanol chemical damage method can be used to establish thin endometrium rat model. Expression of proliferation-related proteins is decreased in thin endometrium animal model, which contributes to the study of thin endometrium-related mechanism and provides guidance for clinical treatment. OBJECTIVE:To explore the changes in vimentin and vascular endothelial growth factor expression in rat modelsof high concentrations of ethanol-induced thin endometrium. METHODS: 0.3 mL 95% ethanol was slowly injected in the right uterus of female Sprague-Dawley rats to establish rat models of thin endometrium. An equal volume of physiological saline was injected into the left uterus as control sides. RESULTS AND CONCLUSION:The thickness of endometrium in the injured uterus was significantly thinner than the control ones. Number of glands reduced, structure became disorder, and vimentin and vascular endothelial growth factor expression markedly diminished. These results suggest that 95% ethanol injury can effectively establish rat models of thin endometrium. Proliferation-related protein expression altered in the thin endometrium.
