Migration and localization of umbilical cord mesenchymal stem cells implanted into brain injury model rats
10.3969/j.issn.2095-4344.2016.01.006
- VernacularTitle:脐带间充质干细胞在颅脑损伤模型鼠体内的迁徙与定位
- Author:
Honglin LIU
;
Zhijun LIU
;
Xiaobing CHEN
;
Wenzhong HU
;
Bingqian DING
- Publication Type:Journal Article
- Keywords:
Umbilical Cord;
Mesenchymal Stem Cel Transplantation;
Craniocerebral Trauma;
Cel Movement;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2016;20(1):31-35
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Choosing an effective means to label and trace the distribution, differentiation and migration of celsin vivo help to further explore the specific mechanism of cels that exert a therapeutic effect. OBJECTIVE:To understand the migration and localization of BrdU-labeled human umbilical cord mesenchymal stem cels in brain injury model rats. METHODS:Human umbilical cord blood samples were obtained, and the isolation of human umbilical cord mesenchymal stem cels was carried out. The primary and passage culture were performed. The phenotype of cels was detected by flow cytometry. Passage 3 human umbilical cord mesenchymal stem cels were labeled using BrdU, and the cel proliferation was detected using MTT method. BrdU-labeled cels were injected into brain injury ratsvia the tail vein. At 14 days after transplantation, brain tissues in the injury region were cut into sections and the migration and location of the umbilical cord mesenchymal stem cels were observed under inverted
fluorescence microscope. RESULTS AND CONCLUSION: Cel surface specific markers CD45 and CD34 were detected by flow cytometry, but the cels could not express CD44, CD105 and CD29. Based on the cel growth curve, the cels came into a conditioning period at 1-3 days of seeding and came into a logarithmic phase at 3-5 days. BrdU-positive cels were visible at the injury region after 14 days, indicating that in the rats, transplanted human umbilical cord mesenchymal stem cels migrated from the peripheral blood to the site of brain injury to achieve the effective repair of injured parts. Cite this article:Liu HL, Liu ZJ, Chen XB, Hu WZ, Ding BQ. Migration and localization of umbilical cord mesenchymal stem cels implanted into brain injury model rats. Zhongguo Zuzhi Gongcheng Yanjiu. 2016;20(1):31-35.
