Feasibility of umbilical cord blood stem cell transplantation for the treatment of diabetic lower limb ischemia
10.3969/j.issn.2095-4344.2016.01.014
- VernacularTitle:脐血干细胞移植治疗糖尿病下肢缺血的可行性
- Author:
Lihua XIE
;
Lin XING
;
Hang ZHENG
- Publication Type:Journal Article
- Keywords:
Diabetes Melitus;
Diabetes Complications;
Diabetic Angiopathies;
Cord Blood Stem Cel Transplantation;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2016;20(1):78-82
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Diabetic lower limb ischemia is prone to involve distal lower limb arteries, and a conventional treatment is often unable to obtain the ideal effect. OBJECTIVE:To investigate the effect and safety of umbilical cord blood stem cel transplantation in the treatment of diabetic lower limb ischemia. METHODS: A diabetic rat model of lower limb ischemia was established, and along the femoral artery, five points
were selected for injection of human umbilical cord mesenchymal stem cel suspension, 20 μL per point. At 1, 2, 4 weeks after transplantation, transcutaneous oxygen pressure, vascular density and vascular endothelial growth factor level in the ischemic region, and incidence of adverse reactions were recorded. RESULTS AND CONCLUSION:At 1, 2 and 4 weeks after transplantation, the transcutaneous oxygen pressure, vascular density and vascular endothelial growth factor level in the ischemic region were found increasing, which were significantly different from those before transplantation (P < 0.05). At different time after transplantation, al animals had no inflammatory reactions such as skin bleeding and dermatitis, and local red, sweling, hot, pain, and had no tumor-like growth in organs. These findings indicate that umbilical cord blood stem cel transplantation can safely and significantly improve symptoms of diabetic lower limb ischemia, which has certain application feasibility. Cite this article:Xie LH, Xing L, Zheng H. Feasibility of umbilical cord blood stem cel transplantation for the treatment of diabetic lower limb ischemia. Zhongguo Zuzhi Gongcheng Yanjiu. 2016;20(1):78-82.
