Biocompatibility of poly-L-lactic acid/hydroxyapatite biomaterial with periodontal ligament cells
10.3969/j.issn.2095-4344.2016.12.009
- VernacularTitle:左旋聚乳酸羟基磷灰石生物材料与牙周膜细胞的生物相容性
- Author:
Ning ZHAO
;
Xianjun ZHANG
;
Wei LI
;
Xinxin DONG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(12):1732-1737
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Hydroxyapatite/poly-L-lactic acid composite can wel improve the brittleness and mechanical properties of the hydroxyapatite. OBJECTIVE:To study the biocompatibility of hydroxyapatite/poly-L-lactic acid composite biomaterials with periodontal ligament cels. METHODS:Passage 4 human periodontal ligament cels at a density of 5×109/L were cultured with hydroxyapatite/poly-L-lactic acid composite as experimental group, and human periodontal ligament cels cultured alone as control group. Cel proliferation was detected within 7 days of culture; alkaline phosphatase activity was detected at 24, 48 and 72 hours of culture; and type I colagen expression was detected at 72 hours of culture. RESULTS AND CONCLUSION:There was no significant difference in the absorbance value of cels between the two groups at 1-7 days of culture. The alkaline phosphatase activity of cels at 48 and 72 hours was significantly higher than that at 24 hours (P < 0.05) in the two groups, but there was no difference between the two groups. The expression of type I colagen had no difference between the two groups. These findings indicate that the hydroxyapatite/poly-L-lactic acid composite biomaterial has good cel compatibility.
