Histological evaluation of type I collagen scaffolds preparde under different dehydrothermal cross-linking conditions in a rat model
10.3969/j.issn.2095-4344.2016.12.011
- VernacularTitle:应用不同物理加强条件的Ⅰ型胶原支架的大白鼠动物模型与组织学分析
- Author:
Cong ZHANG
;
Yanqin ZHANG
;
Spilker MARK
;
Spector MYRON
;
Dengyun LI
;
Yumei LU
;
Heping XU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(12):1745-1752
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:In previous studies, the dehydrothermal cross-linking method was modified by the authors to improve the degradation property of colagen scaffolds. The cross-linking time was increased from 24 to 48 hours, and the cross-linking temperature increased from 105 to 115℃. OBJECTIVE:To verify the anti-degradation ability of colagen scaffolds prepared using the modified dehydrothermal cross-linking method and to obtain the optimal efficacy of the scaffolds on damaged tissue repair and regeneration. METHODS: Highly-purified type I colagen scaffolds with native triple helix structure were prepared and subjected to three different dehydrothermal cross-linking conditions: 105℃ for 24 hours, 105℃ for 48 hours and 115℃ for 24 hours. Material samples, 1 cm×1 cm, were implanted subcutaneously into the rat dorsum. The specimens were harvested at 3 days, 14 days and 42 postoperative days folowed by fixation and histological analysis using hematoxylin-eosin staining. RESULTS AND CONCLUSION:No untoward foreign body and immunological reactions were observed in any groups. In the group of 105℃ for 48 hours, the scaffold retention and degree of pore openness were better than the other two groups at 14 days after scaffold implantation (P < 0.05). These findings indirectly suggest that the anti-degradation ability of colagen scaffolds can be strengthened under certain dehydrothermal cross-linking conditions: the cross-linking time is increased from 24 to 48 hours.
