Stromal cell derived factor-1 promotes the proliferation of bone marrow stem cells:the optimal concentration is 200 μg/L
10.3969/j.issn.2095-4344.2016.10.005
- VernacularTitle:基质细胞衍生因子1促进骨髓干细胞增殖：200μg/L为最佳质量浓度
- Author:
Wei PENG
;
Yuan QIN
;
Chunhui LIAO
;
Songling CHEN
- Publication Type:Journal Article
- Keywords:
Stem Cels;
Bone Marrow;
Cel Proliferation;
Chemokine CXCL12;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2016;20(10):1402-1408
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Stromal cel derived factor-1 is a smal molecular protein with a wide range of biological activity that can cause immune cel chemotaxis, and it also has a chemotactic effect on bone marrow stem cels and periodontal ligament cels.
OBJECTIVE:To investigate the effect of stromal cel derived factor-1 with different concentrations on the proliferation of bone marrow stem cels and to probe the best concentration.
METHODS:Bone marrow stem cels from beagle dogs were culturedin vitro and stimulated by different concentrations of stromal cel derived factor-1 (100, 200, 300 μg/L). MTT was used to detect the influence of stromal cel derived factor-1 on the proliferation of bone marrow stem cels so as to screen the best concentration of stromal cel derived factor-1. Then, stromal cel derived factor-1 at the best concentrations was used to intervene the bone marrow stem cels, and MTT was used again to detect the proliferation of bone marrow stem cels.
RESULTS AND CONCLUSION:Stromal cel derived factor-1 at concentrations of 100, 200, 300 μg/L could promote the proliferation of bone marrow stem cels, and the effect was more notable at 200 and 300 μg/Lbut withno significant difference. Therefore, 200 μg/L was considered to be the best concentration of stromal cel derived factor-1 for intervention of bone marrow stem cels. Compared with the blank control group, 200 μg/L stromal cel derived factor-1 could significantly promote the proliferation of bone marrow stem cels. Taken together, stromal cel derived factor-1 can promote the proliferation of bone marrow stem cels, and its best concentration is 200 μg/L.
