Activation of Wnt signaling pathway in the neuronal differentiation of bone marrow mesenchymal stem cells：a microarray analysis
10.3969/j.issn.2095-4344.2016.10.003
- VernacularTitle:基因芯片技术分析骨髓间充质干细胞神经分化中Wnt信号通路的激活
- Author:
Zhiping LIN
;
Rong ZENG
;
Hao LIN
- Publication Type:Journal Article
- Keywords:
Subject headings:Bone Marrow;
Mesenchymal Stem Cels;
Cel Differentiation;
Neurons;
Microchip Analytical Procedures;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2016;20(10):1389-1395
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:It wil provide a new insight into the future application of bone marrow mesenchymal stem cels in the treatment of spinal cord injury and tissue engineering by studying the effect of activation of Wnt signaling pathway in the neuronal differentiation of bone marrow mesenchymal stem cels.
OBJECTIVE: To detect the expression of related genes by gene chip technology during the neuronal differentiation of bone marrow mesenchymal stem cels.
METHODS:Human bone marrow mesenchymal stem cels were isolated and purified, and passage 5 cels were obtained. GatewayTM technology was used to build lentiviral vectors that was used to transfect Wnt-1 into human bone marrow mesenchymal stem cels. Control, non-transduction and transduction groups were set in this study. Human bone marrow mesenchymal stem cels were then induced to differentiate into neurons. Cel morphology was observed under inverted phase contrast microscope. Gene chip was used to detect the regulation changes and the differential expression of related genes in the Wnt signaling pathway.
RESULTS AND CONCLUSION: Under the scanning electron microscope, the transfected cels were found to have the similar morphology of neuron-like cels. Analysis by the gene chip hybridization technique showed that 3 287 genes were up-regulated and 4 215 genes were down-regulated in the signal pathway. In the Wnt signaling pathway, genes related to the nervous system development and differentiation were up- or down-regulated. It is verified that the Wnt signal pathway is activated via Wnt-1 transduction, and the downstream genes appear to have genetic transcription so as to promote the neuronal differentiation of human bone marrow mesenchymal stem cels.
