Polylysine-modified gamma-Fe2O3 nanoparticle labeling has no effect on neuroblastoma stem cell activation and proliferation
10.3969/j.issn.2095-4344.2016.10.007
- VernacularTitle:多聚赖氨酸修饰γ-Fe2O3纳米颗粒标记不影响神经母细胞瘤干细胞的活化和增殖
- Author:
Zhiyong ZHONG
;
Baojun SHI
;
Hui ZHOU
;
Wenbo WANG
- Publication Type:Journal Article
- Keywords:
Neoplastic Stem Cels;
Neuroblastoma;
Tretinoin;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2016;20(10):1419-1425
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Retinoic acid is the most promising inducer for neuroblastoma minimal residual lesion, and it can induce cel differentiationin vivo, accompanied by reducing tumor cel proliferation.
OBJECTIVE:To study the effect of nanoparticle labeling on biological characteristics of neuroblastoma stem cels, and the role of 13-cis retinoic acid to induce differentiation of neuroblastoma stem cels.
METHODS:Neuroblastoma stem cels were isolated and culturedin vitro using serum-free suspension culture method, labeled with polylysine-modified γ-Fe2O3 nanoparticles and induced in culture medium containing 13-cis retinoic acid. RT-PCR was used to detect the expression of Oct-4 before and after labeling as wel as before and after induction. Immunofluorescence method was used to detect the expression of nestin before and after labeling as wel as before and after induction.
RESULTS AND CONCLUSION:Neuroblastoma stem cels were successfuly cultured in the bone marrow samples from 5 of 20 cases. Polylysine-modified γ-Fe2O3 nanoparticle labeling did no influence the viability and proliferation ability of neuroblastoma stem cels, and also had no effect on Oct-4 mRNA and nestin expression. After cultured in the culture medium containing 13-cis retinoic acid, the cel shape changed and the growth rate slowed down. Moreover, the expression of Oct-4 mRNA and nestin was gradualy reduced. These findings indicate that polylysine-modified gamma-Fe2O3 nanoparticles can be used to label neuroblastoma stem cels, and 13-cis retinoic acid can induce the differentiation of neuroblastoma stem cels.
