Expression of dopamine D1 receptors in hippocampal dentate gyrus in rats with vascular dementia

VernacularTitle:血管性痴呆大鼠海马齿状回区 D1受体的表达
Author: Peng WAN ; Juntao GAO ; Dan WANG ; Shi WANG ; Qinghua JIN
Publication Type:Journal Article
Keywords: vascular dementia; dentate gyrus; receptors,dopamine D1; rats,Sprague-Dawley
From: Journal of Jilin University(Medicine Edition) 2015;(6):1130-1133
CountryChina
Language:Chinese
Abstract: Objective To observe the extracellular content of dopamine (DA)and expression of D1 receptors in hippocampal dentage gyrus (DG)in the model rats with vascular dementia (VD),and to investigate the relationship between them.Methods 12 male SD rats were randomly divided VD group and sham-operation group,and the VD model was prepared by permanent bilateral carotid occlusion.The extracellular content of DA in the DG was determined by in vivo microdialysis and HPLC,and the expression of D1 receptors was measured by immunehisto-chemistry.Results The DA content in the DG of the rats in VD group was lower than that in sham-operation group (P <0.05).The number of D1 receptor-positive cells in the DG hilus in VD group was increased compared with sham-operation group (P < 0.05),whereas the expression of D1 receptor in DG granule cell layer did not change (P > 0.05).Conclusion The DA content in the hippocampal DG is decreased in the VD rats,and its function may be compensated by the up-regulation of D1 receptors in the DG hilus.