Study on Decoloration Process for Crude Polysaccharides from Cordyceps militaris Link. by Macroporous Resin

VernacularTitle:大孔树脂对蛹虫草粗多糖的脱色工艺研究
Author: Jun WU ; Bo LIU ; Weiling LIANG ; Qinde ZHANG
Publication Type:Journal Article
Keywords: Macroporous resin; Cordyceps militaris Link./isolation&purification; Crude polysaccharides/analysis; Decoloration
From: Journal of Guangzhou University of Traditional Chinese Medicine 2015;(4):739-744
CountryChina
Language:Chinese
Abstract: Objective To study the optimum decoloration technological conditions of crude polysaccharides from Cordyceps militaris Link. (PCM) by macroporous resin. Methods With the decolorization rate and retention rate of PCM as indexes, static adsorption factor experiment was used for the optimization of seven kinds of macroporous resin. And then the optimum conditions of decolorization of PCM were investigated by the static/dynamaic adsorption single-factor experiments. Results D941 macroporous resin is the best. The optimum decoloration technological conditions were as follows:initial concentration of the sample was 50 mg/mL, pH value was 5.5, operating temperature was 45℃, the flow rate of dynamaic adsorption was 2.5 BV/h, and the sample solution volume was 5 bed volume per hour. Under the optimal conditions, decolorization rate and retention rate of PCM were ( 93.9 ± 3.4) % and (91.7 ± 2.2) %, respectively. Conclusion The resin D941 is suitable for the decoloration of PCM. The optimized method is feasible and reliable, having the prospects for popularizing.