Study on Callus Induction in Explant of Aquiliaria sinensis Root

VernacularTitle:白木香根外植体愈伤组织诱导研究
Author: Yanfengyang JIANG ; Chao LIN ; Ruoting ZHAN ; Xinye MA
Publication Type:Journal Article
Keywords: Aquilaria sinensis/growth&development; Callus induction; Roots
From: Journal of Guangzhou University of Traditional Chinese Medicine 2015;(4):751-754
CountryChina
Language:Chinese
Abstract: Objective To study the conditions of callus induction with the roots of Aquilaria sinensis as explants. Methods Two sources of roots of Aquiliaria sinensis were selected as the explants. The effects of sterilization methods and the combination of different concentrations of phytohormones on callus induction were evaluated. Results When Aquiliaria sinensis root seedling was sterilized in 0.01mg/mL HgCl2 solution for 3 minutes, the sterilized effect was the best. The optimal callus induction medium was MS+0.1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) +0.1 mg/L 6-benzylaminopurine (6-BA). Aseptic Aquiliaria sinensis root seedling cultivated in callus induction medium containing MS+1.0 mg/L naphthalene acetic acid ( NAA) +0.8 mg/L 6-BA achieved the highest callus induction rate. Conclusion Callus can be induced from two sources of Aquilaria sinensis roots. The induction rate of callus is lower when the explant root seedling is cultivated using 2,4-D alone as inducer, and is increased when used together with 6-BA.