Prevention of Retinal Vascular Endothelium and Radiation-induced Rat Optic Nerve Damage with Orifices-dredging and Stasis-removing Therapy
10.13359/j.cnki.gzxbtcm.2015.04.030
- VernacularTitle:通窍化瘀法对大鼠视网膜血管内皮和视神经放射性损伤的预防作用
- Author:
Jianping JI
;
Xianhua CHENG
;
Lihua LIAN
;
Hong LIU
;
Lei CHEN
;
Zhiying LI
- Publication Type:Journal Article
- Keywords:
Radiation-induced optic neuropathy/TCD therapy;
Orifices-dredging and stasis-removing therapy;
Optic nerve/ultrastructure;
Disease models,animal;
Rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2015;(4):715-719
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the preventive effect of orifices-dredging and stasis-removing therapy on retinal vascular endothelium and optic nerve damage induced by radiation in rats. Methods Thirty-six SD rats were randomly divided into 3 groups, namely blank control group, model group and therapy group. Rats in the model group and therapy group were exposed to X-ray radiation once a week for three weeks. Two weeks before the radiation, therapy group was pretreated with intraperitoneal injection of Astragalus injection ( 4.0 g/kg) , Ligustrazine Hydrochloride injection ( 15 mg/kg) and Xingnaojing injection ( 2.5 mL/kg) once a day, blank control group and model group were given intraperitoneal injection of same volume of saline once a day. Before radiation, one day, and 2, 4 and 6 weeks after radiation, we detected the blood concentrations of endothelin-1 ( ET-1) and Von willebrand factor (vWF) in all of the rats by enzyme-linked immunosorbent assay (ELISA) , and analyzed the dynamic changes and intergroup difference. Demyelination of optic nerve was observed under transmission electron microscope, and demyelination percentage was counted 2, 4, and 6 weeks after irradiation. Results Compared with the blank control group, the blood concentrations of ET-1 and vWF in model group were increased after irradiation (P<0.05) , and the peak value appeared on the fourth week after irradiation. Compared with the model group, the blood concentrations of ET-1 and vWF in the therapy group were decreased weeks 2 after irradiation ( P<0.05) . The demyelination percentage of optic nerve was significantly higher in the model group than that in the blank control group (P<0.05) 2, 4, and 6 weeks after irradiation. Compared with the model group, the demyelination percentage of optic nerve in therapy group showed insignificant changes 2 and 4 weeks after irradiation (P>0.05) , but was decreased obviously 6 weeks after irradiation (P<0.05) . Conclusion Pretreatment with orifices-dredging and stasis-removing therapy can decrease the blood concentrations of ET-1 and vWF in the radiation-induced retinal damage rats, and can reduce the demyelination of optic nerve in irradiated rats.
