Influence of tripterygium glycosides in BMP-2 expression in fibroblasts of patients with ankylosing spondylitis
10.13481/j.1671-587x.20140613
- VernacularTitle:雷公藤多苷对强直性脊柱炎患者成纤维细胞中BMP-2表达的影响
- Author:
Hongchang ZHANG
;
Ying ZHANG
;
Mingxin LIU
;
Yan TANG
;
Pengfei ZHANG
;
Zhi PAN
- Publication Type:Journal Article
- Keywords:
tripterygium glycosides;
ankylosing spondylitis;
bone morphogenetic protein-2;
fibroblasts
- From:
Journal of Jilin University(Medicine Edition)
2014;(6):1187-1191
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of tripterygium glycosides (LGTDG)in the bone morphogenetic protein(BMP)signal transduction pathway and BMP-2 expression,and to clarify the mechanism of anti-ankylosing spondylitis (AS)ossification of LGTDG.Methods The in vitro cultured AS fibroblasts were divided into control group and 0.5,1.0,1.5,2.0 mg·L-1 LGTDG groups.The alkaline phosphatase (ALP)activities of the cells and optimal drug concentrations in various groups were detected;CCK-8 assay was used to detect the proliferation rate of the cells in 1.0 mg· L-1 LGTDG group;the biochemical tests were performed to quantitatively detect the BMP-2 expression levels in control group and 1.0 mg· L-1 LGTDG group;Western blotting method was used to determine the BMP-2 and Cbfal protein expressions. Results The ALP activities in LGTDG groups were lower than that in control group,especially in 1.0 mg·L-1 LGTDG group (P<0.01).The CCK-8 assay results showed that the proliferation rate of AS fibroblasts in 1.0 mg·L-1 LGTDG group was significantly low than that in control group(P<0.01),the proliferation rate reached the peak at the 4th day after LGTDG treatment and entered into the plateau phase,then the proliferation rate of the cells was decreased.The biochemical assay and Western blotting results indicated that the protein expression levels of BMP-2 in 1.0 mg·L-1 LGTDG group was significantly lower than that in control group (P<0.01).Conclusion LGTDG can effectively inhibit the BMP-2 expression in AS fibroblasts and delay the cells to differentiate into the osteoblasts and lead to AS ossification by BMP signal transduction pathway.
