Effects of Yishen Tongluo Decoction on Renal Transforming Growth Factor-β1 and CollagenⅣmRNA Expression in Membranous Nephropathy Rats
10.13359/j.cnki.gzxbtcm.2014.06.017
- VernacularTitle:益肾通络方对膜性肾病大鼠肾组织中转化生长因子-β1、Ⅳ型胶原mRNA表达的影响
- Author:
Cong QIN
;
Huaping FAN
;
Xiuxia ZHANG
;
Zhongshan BAI
;
Qian ZHANG
;
Xiaoya WANG
;
Jinchuan TAN
- Publication Type:Journal Article
- Keywords:
Yishen Tongluo Decoction/pharmacology;
Membranous nephropathy/TCD therapy;
Kidney/pathology;
Kidney/ultrastructure;
Gene expression regulation;
Disease models,animal;
Rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2014;(6):928-931
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Yishen Tongluo Decoction (YTD) on the renal mRNA expression of transforming growth factor-β1 ( TGF-β1) and collagen Ⅳ ( ColⅣ) in membranous nephropathy ( MN) rats. Methods SD rats were randomly divided into normal group, model group, benazepril group (in the dosage of 10 mg·kg-1·d-1) , YTD group ( in the dosage of 20 g·kg-1·d-1) . The rats in various groups were given intragastric administration of corresponding agents. At the end of the fourth week, 24-hour urinary protein quantity, albumin ( ALB) , total protein ( TP) , triglyceride ( TG) , total cholesterol ( TC) , blood urea nitrogen ( BUN) , and creatinine (Cr) levels were observed. The mRNA expression levels of TGF-β1 and ColⅣ in renal tissue of rats were detected by immunofluorescence method, electron microscopy and real-time polymerase chain reaction (PCR) method. Results In the model group, urinary protein quantity in rats was increased, serum levels of TP and ALB were significantly lowered, serum levels of TC and TG were significantly increased, renal pathological changes were present, and mRNA expression levels of TGF-β1 and ColIV in renal tissue were up-regulated (P<0.05 compared with normal group). Compared with the model group, 24-hour urinary protein quantity, TC and TG levels were significantly lowered, TP and ALB levels were significantly increased, rat renal injury was relieved, mRNA expression levels of TGF-β1 and ColIV in renal tissue were down-regulated in the treatment groups ( P<0.05) . However, the differences between benazepril group and YTD group were insignificant ( P>0.05) . Conclusion The therapeutic mechanism of YTD for MN is probably related with the inhibition of mRNA expression of TGF-β1 and ColⅣin renal tissue.
