Gene Chip Screening of Related Genes of Shenqi Compound Recipe in Preventing Diabetic Skeletal Muscle Disease
10.13359/j.cnki.gzxbtcm.2014.06.021
- VernacularTitle:用基因芯片筛选参芪复方保护糖尿病骨骼肌的相关基因
- Author:
Haiyan ZHU
;
Hong GAO
;
Baogen GUO
;
Fei ZHOU
;
Chunguang XIE
- Publication Type:Journal Article
- Keywords:
Type 2 diabetes mellitus/TCD therapy;
Shenqi Compound Recipe/pharmacology;
Macroangiopathy;
Differentiatly expressed genes;
Abdominal aorta/pathology;
Gastrocnemius muscle/pathology;
Disease models,animal;
Mice
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2014;(6):944-948
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation of diabetic skeletal muscle disease with macroangiopathy, and to explore the related genes of Shenqi Compound Recipe (SCR) in preventing and treating diabetic skeletal muscle disease by using gene chip technique, thus to reveal the molecular mechanism. Methods KKAy mice were fed with water containing nitri oxide synthase inhibitor of Nω-nitro-L-arginine methyl ester ( L-NAME) and high fat diet to induce the macroangiopathy complicated with type 2 diabetes. The experimental animals were divided into normal c57BL/GJ group, KKAy group, model group, SCR group (in the dosage of 14.4 g·kg-1·d-1) and rosiglitazone group ( in the dosage of 1.33 mg·kg-1·d-1) , 15 in each group. The medication groups were administered the corresponding agents for 8 consecutive weeks just as the modeling began. During the experiment period, blood glucose was monitored. At the end of the experiment, the abdominal aorta and skeletal muscle of mice were taken out for the observation of morphological changes, and differentially expressed genes of skeletal muscle between SCR group and model group, and between model group and KKAy group were detected by gene chip technique. Results SCR had an effect on relieving the atrophy, edema, fracture, and inflammatory changes in the skeletal muscle. There were 198 genes differentially expressed between model group and KKAy group, including 119 up-regulated genes and 79 down-regulated genes. There were 70 genes differentially expressed between SCR group and model group, including 33 up-regulated genes and 37 down-regulated genes. In the two comparison groups, 7 genes ( Celsr2, Rilpl1, Dlx6as, 2010004M13Rik, Anapc13, Gm6097, Ddx39b) showed reversed differential expression. Conclusion Diabetic skeletal muscle disease is associated with macroangiopathy. SCR has preventive effect on diabetic skeletal muscle lesion, and the mechanism may be related to the regulation of Celsr2, Rilpl1, Dlx6as, 2010004M13Rik, Anapc13, Gm6097, Ddx39b gene expression.
