Establishment and evaluation of method to collect rat gingival crevicular fluid by using absorbent paper points
10.13481/j.1671-587x.20140524
- VernacularTitle:大鼠龈沟液吸潮纸尖采集方法的建立及其评价
- Author:
Zhigang ZUO
;
Min HU
;
Shan LIU
;
Zhimin LI
;
Huan JIANG
;
Hongfa LI
- Publication Type:Journal Article
- Keywords:
gingival crevicular fluid;
absorbent paper points;
protein analysis;
rats,Wistar
- From:
Journal of Jilin University(Medicine Edition)
2014;(5):1028-1032
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and evaluate the method to collect the rat gingival crevicular fluid (GCF)by using absorbent paper points, and to lay foundation for analysis on GCF.Methods 20 healthy male rats were selected and randomly divided into GCF group and saliva group.The GCF of the right upper molar gingival trough of the rats in GCF group and the saliva of the rats in saliva group were collected by using 1 5# absorbent paper points.The SDS-PAGE analysis and abundance detection were applied to analyze the protein bands of the samples in two groups.Results The SDS-PAGE analysis identified the proteins at 77 000,66 000,55 000,51 000,and 28 000,especially 66 000 in GCF group.While saliva group had lower brightness protein bands at 66 000,60 000, and 48 000.The data of protein abundance of 66 000 between two groups had statistically significant difference (P<0.05).Conclusion The number and types of the protein bands are different between GCF Group and saliva group,so using 15# absorbent paper points can collect the rat GCF successfully.
