Screening of Stilbene Glucosides Biosynthesis-related Genes in Polygonum multiflorum Thunb. by mRNA Differential Display Reverse Transcription Polymerase Chain Reaction
10.13359/j.cnki.gzxbtcm.2014.05.025
- VernacularTitle:mRNA差异显示技术筛选何首乌中二苯乙烯苷合成相关基因
- Author:
Jiewen LI
;
Wei ZHAO
;
Shujin ZHAO
- Publication Type:Journal Article
- Keywords:
Polygonum multiflorum Thunb.;
Stilbene glucoside;
Differential display reverse transcription polymerase chain reaction;
Gene expression difference
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2014;(5):799-803
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen and clone the genes related to stilbene glucosides biosynthesis in Polygonum multiflorum Thunb. Methods The differentially expressed genes in the root, stem and leaf of Polygonum multiflorum which have different contents of stilbene glucosides were screened by differential display reverse transcription polymerase chain reaction (DDRT-PCR). After pMD19-T carrier was inserted into the obtained differential genes for sequencing and comparison, the gene function was analyzed. Results Fifty-one differentially expressed cDNA fragments were found. Of them, 9 were used for the identification by semi-quantitative PCR. The identification results presented 3 positive fragments, one fragment was specifically expressed in the stem and leaf of Polygon-um multiflorum Thunb., sharing high homology with glycine dehydrogenase, and 2 were specifically expressed in the root of Polygonum multiflorum Thunb., having high homology with enoyl-CoA hydratase and aminopeptidase N, respectively. Conclusion Three homologous gene sequences obtained through DDRT-PCR provide a basis for the further study of biosynthetic pathway of stilbene glucoside from Polygonum multiflorum Thunb..
