Neuroprotective effect of neural stem cells modified by glial-derived neurotrophic factor on cerebral apoplexy
10.3969/j.issn.2095-4344.2015.45.021
- VernacularTitle:脑外源性神经因子基因修饰神经干细胞对脑卒中的神经保护机制
- Author:
Zhonghua WU
- Publication Type:Journal Article
- Keywords:
Tissue Engineering;
Stem Cels;
Stroke
- From:
Chinese Journal of Tissue Engineering Research
2015;(45):7331-7336
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Glial-derived neurotrophic factor has a specific effect on brain neurons, and is an important neurotrophic factor in the treatment of cerebral apoplexy. OBJECTIVE:To investigate the neuroprotective effect of glial-derived neurotrophic factor gene modified neural stem cels on rat cerebral apoplexy. METHODS:The recombinant human plasmid pAdEasy-l-pAdTrackCMV was constructed, and the neural stem cels were isolated and cultured from the cortex of neonatal rats. The neural stem cels were transfected with the recombinant adenovirus of glial-derived neurotrophic factor, and the cel suspension was injected into the right brain ventricle of rats with transient cerebral ischemia (2 hours). Meanwhile, neural stem cel transplantation group and control group were set up. RESULTS AND CONCLUSION:Compared with the neural stem cel transplantation group, the modified neurological severity score of combined transplantation group was reduced significantly 2 and 3 weeks after reperfusion, and the area of cerebral ischemia injury was also significantly decreased at 7 days after reperfusion (P< 0.05). The number of neural stem cels in the neural stem cel transplantation group was significantly less than that in the combined transplantation group (P < 0.05). The expression of Syn, PSD-95 proteins in the two transplantation groups, especialy in the combined transplantation group, was higher than that of the control group (P < 0.05). However, there was no significant difference between the two transplantation groups (P > 0.05). The results show that the neural stem cels modified by glial-derived neurotrophic factor can play a better role in the neuroprotection against cerebral apoplexy in rats, and the effect is better than that of simple neural stem cels.
